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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/09/2014 |
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Data da última atualização: |
29/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P. |
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Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, BIOAGRO - UFV; TETSU SAKAMOTO, UFMG; RICARDO DIÓGENES DIAS SILVEIRA; PATRICIA FERNANDA ZAMBUSSI-CARVALHO, UFG; MARISTELA PEREIRA, UFG; GEORGIOS JOANIS PAPPAS JUNIOR, UNB; MARCOS MOTA DO CARMO COSTA, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; WENDELL JACINTO PEREIRA; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 32, p. 438-451, 2014. |
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DOI: |
10.1007/s11105-013-0651-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. MenosDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly thos... Mostrar Tudo |
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Thesagro: |
Deficiência hídrica; Feijão; Phaseolus vulgaris. |
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Thesaurus Nal: |
Abiotic stress; Beans; Water stress. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180004/1/Muller2014-Article-DifferentiallyExpressedGenesDu.pdf
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Marc: |
LEADER 02986naa a2200325 a 4500
001 1994817
005 2024-04-29
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s11105-013-0651-7$2DOI
100 1 $aMÜLLER, B. S. de F.
245 $aDifferentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.$h[electronic resource]
260 $c2014
520 $aDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding.
650 $aAbiotic stress
650 $aBeans
650 $aWater stress
650 $aDeficiência hídrica
650 $aFeijão
650 $aPhaseolus vulgaris
700 1 $aSAKAMOTO, T.
700 1 $aSILVEIRA, R. D. D.
700 1 $aZAMBUSSI-CARVALHO, P. F.
700 1 $aPEREIRA, M.
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aCOSTA, M. M. do C.
700 1 $aGUIMARÃES, C. M.
700 1 $aPEREIRA, W. J.
700 1 $aBRONDANI, C.
700 1 $aVIANELLO-BRONDANI, R. P.
773 $tPlant Molecular Biology Reporter, Athens$gv. 32, p. 438-451, 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/08/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
QUILICI, L. S.; SILVA-PEREIRA, I.; ANDRADE, A. C.; ALBUQUERQUE, F. C.; BRIGIDO, M. M.; MARANHÃO, A. Q. |
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Afiliação: |
L. S. QUILICI, UNB; I. SILVA-PEREIRA, UNB; ALAN CARVALHO ANDRADE, CENARGEN; F. C. ALBUQUERQUE, UNB; M. M. BRIGIDO, UNB; A. Q. MARANHÃO, UNB. |
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Título: |
A minimal cytomegalovirus intron A variant can improve transgene expression in different mammalian cell lines. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Biotechnology Letters, v. 35, p. 21-27, 2013. |
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Idioma: |
Inglês |
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Palavras-Chave: |
CMV promoter; Intron A; Recombinant antibody. |
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Thesaurus NAL: |
gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179295/1/Quilici2013-Article-AMinimalCytomegalovirusIntronA.pdf
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Marc: |
LEADER 00673naa a2200217 a 4500
001 1963723
005 2023-03-07
008 2013 bl uuuu u00u1 u #d
100 1 $aQUILICI, L. S.
245 $aA minimal cytomegalovirus intron A variant can improve transgene expression in different mammalian cell lines.$h[electronic resource]
260 $c2013
650 $agene expression
653 $aCMV promoter
653 $aIntron A
653 $aRecombinant antibody
700 1 $aSILVA-PEREIRA, I.
700 1 $aANDRADE, A. C.
700 1 $aALBUQUERQUE, F. C.
700 1 $aBRIGIDO, M. M.
700 1 $aMARANHÃO, A. Q.
773 $tBiotechnology Letters$gv. 35, p. 21-27, 2013.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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